One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays

This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in...

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Detaylı Bibliyografya
Yazar: Song, Seo Woo (Yazar)
Müşterek Yazar: SpringerLink (Online service)
Materyal Türü: e-Kitap
Dil:İngilizce
Baskı/Yayın Bilgisi: Singapore : Springer Nature Singapore : Imprint: Springer, 2019.
Edisyon:1st ed. 2019.
Seri Bilgileri:Springer Theses, Recognizing Outstanding Ph.D. Research,
Konular:
Biomedical engineering.
Bioinformatics.
Soft condensed matter.
Microtechnology.
Microelectromechanical systems.
Industrial microbiology.
Biomedical Engineering and Bioengineering.
Computational and Systems Biology.
Soft and Granular Matter.
Microsystems and MEMS.
Industrial Microbiology.
Online Erişim:Full-text access
OPAC'ta görüntüle
İçindekiler:
  • Abstract
  • Table of Contents
  • List of Tables
  • List of Figures
  • Chapter 1. Introduction
  • 1.1. High-Throughput Small-Volume Bioassays
  • 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms
  • 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs)
  • Chapter 2. System Development
  • 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions
  • 2.2. Chip and Jig Development
  • 2.3. Preparation of DLPs Library
  • 2.4. Decoding Microparticles
  • 2.5. Statistical Analysis for Duplications
  • Chapter 3. Application: Screening of Sequential Drug Combinations
  • 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways
  • 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform
  • 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip
  • 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC)
  • Chapter 4. Conclusion and Discussion
  • Bibliography.

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