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Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases
This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully s...
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| Format: | e-Book |
| Language: | English |
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Cham :
Springer International Publishing : Imprint: Springer,
2016.
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| Edition: | 1st ed. 2016. |
| Series: | Springer Theses, Recognizing Outstanding Ph.D. Research,
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| Online Access: | Full-text access View in OPAC |
Table of Contents:
- Introduction
- Materials and Methods
- Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains
- Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains
- Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology
- Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.